Studies on new acid proteases from Scytalidium lignicolum M-133. V. Action of Scytalidium lignicolum acid proteases on insulin B-chain.
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منابع مشابه
Nucleotide sequence of the gene encoding the precursor protein of pepstatin insensitive acid protease B, scytalidopepsin B, from Scytalidium lignicolum.
A chromosomal DNA of Scytalidium lignicolum was digested with Sau3AI. The digest was self-ligated and amplified by inverse PCR procedure using primers designed based on the nucleotide sequences of up- and down-stream regions of an intron present in the scytalidopepsin B gene. Analysis of the nucleotide sequence of PCR product (700 bp) showed that the enzyme is synthesized as a precursor protein...
متن کاملNucleic acid aptamers against proteases.
Proteases are potential or realized therapeutic targets in a wide variety of pathological conditions. Moreover, proteases are classical subjects for studies of enzymatic and regulatory mechanisms. We here review the literature on nucleic acid aptamers selected with proteases as targets. Designing small molecule protease inhibitors of sufficient specificity has proved a daunting task. Aptamers s...
متن کاملScytalidium dimidiatum fungal endophthalmitis.
Fungal endophthalmitis after surgery or trauma is uncommon. 1-7 We are not aware of any report in the literature ofScytalidium dimidiatum (under the name S synanamorph subspecies of Hendersonula toruloidea) causing eye infection.>" This paper reports a severe case of S dimidiatum -endopthalmitis following trauma. Despite intraocular amphotericin B, miconazole, topical natamycin, and extensive s...
متن کاملHomology among acid proteases: comparison of crystal structures at 3A resolution of acid proteases from Rhizopus chinensis and Endothia parasitica.
The molecular structures of two fungal acid proteases at 3 A resolution have been compared, and found to have similar secondary and tertiary folding. These enzymes are bilobal and have a pronounced cleft between the two lobes. This cleft has been identified as the active site region from inhibitor binding studies. The results of the comparison are discussed in terms of homology among the acid p...
متن کاملIn vitro activity of amphotericin B, itraconazole, voriconazole, posaconazole, caspofungin and terbinafine against Scytalidium dimidiatum and Scytalidium hyalinum clinical isolates.
OBJECTIVES The objective of this work was to evaluate the in vitro activity of amphotericin B, itraconazole, voriconazole, posaconazole, caspofungin and terbinafine against clinical isolates of Scytalidium dimidiatum and Scytalidium hyalinum. METHODS Microdilution testing was performed in accordance with the CLSI M38-A method on 17 S. dimidiatum and 15 S. hyalinum clinical isolates. RESULTS...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1976
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.40.1221